Adjusting Bright Field Images

Processing a bright field image mainly consists of adjusting the contrast and the brightness to improve the quality of the image. Cytosketch provides tools that make adjusting these images substantially faster, easier and more consistent.

The adjustments to the image are non-destructive, this means that the adjustments do not degrade the quality of the image and it means that you can always reset the adjustments to the original values.

This section describes the various ways to adjust bright field images in Cytosketch.

Table of Content

Bright Field Image Controls Overview

This section provides a quick overview of the controls that are available in the Adjust Image box when adjusting bright field images. Move the mouse over the screen shot below to see a short description of the individual controls or over the description to see the corresponding controls on the screen shot.

Bright Field Controls BF image contrast brightness convert to merged convert to channel reset auto adjust apply
  1. Indicates the selected image as being a bright field image.
  2. Controls to adjust the contrast of the selected images.
  3. Controls to adjust the brightness of the selected images.
  4. Button to convert the bright field image to a merged fluorescent image.
  5. Button to convert the bright field image to a single fluorescent channel image.
  6. Resets the adjustment values back to the original values.
  7. Automatically adjusts the brightness and the contrast of the selected images.
  8. Applies the current adjustment values to the rest of the selected images.

Automatically Adjusting Images

It's easy - select the images to adjust and click the Auto button Auto in the Adjust Image box or press Ctrl + G simultaneously.

Adjusting Contrast

Select the image to adjust and then use the controls in the Adjust Image box to adjust the contrast.

Contrast Controls

Automatically Adjusting Brightness

  1. Select the image to adjust, go to Image and then select Auto Brightness or press Ctrl + B simultaneously.
  2. To fine tune the brightness use the controls in the Adjust Image box.
  3. Brightness Controls

How to Get Back to the Original Image

  1. Select the image to reset.
  2. Click Reset in the Adjust Image box and the adjustments values are reset to the original values.

Converting a Bright Field Image into a Merged Fluorescent Image

Converting a bright field image to a merged fluorescent image is useful if you want to adjust each channel individually or if you have accidently imported your fluorescent image as a bright field image.

  1. Select the image.
  2. Click the Merged button adjust image boxin the Adjust Image box.
  3. convertToMerged

Tip:

For an overview of the fluorescent image control box have a look at Adjusting Fluorescent Images.

Converting a Bright Field Image into a Single Fluorescent Channel

Converting a bright field image to a single fluorescent channel is useful if you have accidently imported your single channel image as a bright field image.

  1. Select the images by using the selection tool Selection Tool.
  2. Click the Channel button channel in the Adjust Image box. The channel with the brightest color is extracted from the bright field image.
  3. convertToChannel

Tip:

Use the Split button split button in the tool bar to split the image into separate channels while retaining the bright field image.